Behavior and preservation of an in vitro collection of European aspen in Spain.
Identifieur interne : 003C38 ( Main/Exploration ); précédent : 003C37; suivant : 003C39Behavior and preservation of an in vitro collection of European aspen in Spain.
Auteurs : M T Martin [Espagne] ; H E Pedranzani ; R. Sierra De GradoSource :
- Biocell : official journal of the Sociedades Latinoamericanas de Microscopia Electronica ... et. al [ 0327-9545 ] ; 2007.
Descripteurs français
- KwdFr :
- MESH :
- composition chimique : Milieux de culture.
- croissance et développement : Arbres, Populus, Structures de plante.
- Clones cellulaires, Conservation biologique, Espagne, Techniques de culture.
- Wicri :
- geographic : Espagne.
English descriptors
- KwdEn :
- MESH :
- chemical , chemistry : Culture Media.
- geographic : Spain.
- growth & development : Plant Structures, Populus, Trees.
- Clone Cells, Culture Techniques, Preservation, Biological.
Abstract
An in vitro collection has been established with selected European aspen from Palencia province (Spain). Currently, this collection includes 32 high quality clones, selected for their good bearing and healthy state. Most of them belong to different discrete local populations. Populus tremula L. was propagated in proliferation Aspen Culture Medium; they required subculture every 3 months. The purpose of this study was, therefore, to select a medium which allows the maintenance of 32 clones for a period longer than 3 months without subculture and to observe the behavior of those clones in 15 different culture medium compositions. Seven nodal cutting stem explants from each clone were cultured in parallel in the different media. One and three months after setting on the stem explants, the number and the size of shoots, the root size, the presence or absence of callus and the survival, were evaluated. The survival was monthly recorded during 8 months. Taking into account the explant development, four media were proposed for collection preservation. One of them, Ga, with a reduction of salts, sucrose, 6-benzoaminopurine, omitting adenine sulphate and 1-naphthalene acetic acid, is the most economical. Behavior observations of the 32 clones in the 15 medium compositions showed the influence of the genotype of the clones.
PubMed: 17665638
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
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Producción Vegetal y Recursos Forestales, Escuela Técnica Superior Ingenierías Agrarias, Universidad de Valladolid, Avda. de Madrid, 57, Palencia E-34071</wicri:regionArea><placeName><region nuts="2" type="communauté">Castille-et-León</region></placeName></affiliation></author><author><name sortKey="Pedranzani, H E" sort="Pedranzani, H E" uniqKey="Pedranzani H" first="H E" last="Pedranzani">H E Pedranzani</name></author><author><name sortKey="Sierra De Grado, R" sort="Sierra De Grado, R" uniqKey="Sierra De Grado R" first="R" last="Sierra De Grado">R. 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Producción Vegetal y Recursos Forestales, Escuela Técnica Superior Ingenierías Agrarias, Universidad de Valladolid, Avda. de Madrid, 57, Palencia E-34071, Spain. marvilte@itacyl.es</nlm:affiliation><country xml:lang="fr">Espagne</country><wicri:regionArea>Dpt. Producción Vegetal y Recursos Forestales, Escuela Técnica Superior Ingenierías Agrarias, Universidad de Valladolid, Avda. de Madrid, 57, Palencia E-34071</wicri:regionArea><placeName><region nuts="2" type="communauté">Castille-et-León</region></placeName></affiliation></author><author><name sortKey="Pedranzani, H E" sort="Pedranzani, H E" uniqKey="Pedranzani H" first="H E" last="Pedranzani">H E Pedranzani</name></author><author><name sortKey="Sierra De Grado, R" sort="Sierra De Grado, R" uniqKey="Sierra De Grado R" first="R" last="Sierra De Grado">R. Sierra De Grado</name></author></analytic><series><title level="j">Biocell : official journal of the Sociedades Latinoamericanas de Microscopia Electronica ... et. al</title><idno type="ISSN">0327-9545</idno><imprint><date when="2007" type="published">2007</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Clone Cells (MeSH)</term><term>Culture Media (chemistry)</term><term>Culture Techniques (MeSH)</term><term>Plant Structures (growth & development)</term><term>Populus (growth & development)</term><term>Preservation, Biological (MeSH)</term><term>Spain (MeSH)</term><term>Trees (growth & development)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Arbres (croissance et développement)</term><term>Clones cellulaires (MeSH)</term><term>Conservation biologique (MeSH)</term><term>Espagne (MeSH)</term><term>Milieux de culture (composition chimique)</term><term>Populus (croissance et développement)</term><term>Structures de plante (croissance et développement)</term><term>Techniques de culture (MeSH)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Culture Media</term></keywords><keywords scheme="MESH" type="geographic" xml:lang="en"><term>Spain</term></keywords><keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Milieux de culture</term></keywords><keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Arbres</term><term>Populus</term><term>Structures de plante</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>Plant Structures</term><term>Populus</term><term>Trees</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Clone Cells</term><term>Culture Techniques</term><term>Preservation, Biological</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Clones cellulaires</term><term>Conservation biologique</term><term>Espagne</term><term>Techniques de culture</term></keywords><keywords scheme="Wicri" type="geographic" xml:lang="fr"><term>Espagne</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">An in vitro collection has been established with selected European aspen from Palencia province (Spain). Currently, this collection includes 32 high quality clones, selected for their good bearing and healthy state. Most of them belong to different discrete local populations. Populus tremula L. was propagated in proliferation Aspen Culture Medium; they required subculture every 3 months. The purpose of this study was, therefore, to select a medium which allows the maintenance of 32 clones for a period longer than 3 months without subculture and to observe the behavior of those clones in 15 different culture medium compositions. Seven nodal cutting stem explants from each clone were cultured in parallel in the different media. One and three months after setting on the stem explants, the number and the size of shoots, the root size, the presence or absence of callus and the survival, were evaluated. The survival was monthly recorded during 8 months. Taking into account the explant development, four media were proposed for collection preservation. One of them, Ga, with a reduction of salts, sucrose, 6-benzoaminopurine, omitting adenine sulphate and 1-naphthalene acetic acid, is the most economical. Behavior observations of the 32 clones in the 15 medium compositions showed the influence of the genotype of the clones.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">17665638</PMID><DateCompleted><Year>2007</Year><Month>08</Month><Day>24</Day></DateCompleted><DateRevised><Year>2009</Year><Month>11</Month><Day>19</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0327-9545</ISSN><JournalIssue CitedMedium="Print"><Volume>31</Volume><Issue>1</Issue><PubDate><Year>2007</Year></PubDate></JournalIssue><Title>Biocell : official journal of the Sociedades Latinoamericanas de Microscopia Electronica ... et. al</Title><ISOAbbreviation>Biocell</ISOAbbreviation></Journal><ArticleTitle>Behavior and preservation of an in vitro collection of European aspen in Spain.</ArticleTitle><Pagination><MedlinePgn>41-9</MedlinePgn></Pagination><Abstract><AbstractText>An in vitro collection has been established with selected European aspen from Palencia province (Spain). Currently, this collection includes 32 high quality clones, selected for their good bearing and healthy state. Most of them belong to different discrete local populations. Populus tremula L. was propagated in proliferation Aspen Culture Medium; they required subculture every 3 months. The purpose of this study was, therefore, to select a medium which allows the maintenance of 32 clones for a period longer than 3 months without subculture and to observe the behavior of those clones in 15 different culture medium compositions. Seven nodal cutting stem explants from each clone were cultured in parallel in the different media. One and three months after setting on the stem explants, the number and the size of shoots, the root size, the presence or absence of callus and the survival, were evaluated. The survival was monthly recorded during 8 months. Taking into account the explant development, four media were proposed for collection preservation. One of them, Ga, with a reduction of salts, sucrose, 6-benzoaminopurine, omitting adenine sulphate and 1-naphthalene acetic acid, is the most economical. Behavior observations of the 32 clones in the 15 medium compositions showed the influence of the genotype of the clones.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Martin</LastName><ForeName>M T</ForeName><Initials>MT</Initials><AffiliationInfo><Affiliation>Dpt. Producción Vegetal y Recursos Forestales, Escuela Técnica Superior Ingenierías Agrarias, Universidad de Valladolid, Avda. de Madrid, 57, Palencia E-34071, Spain. marvilte@itacyl.es</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Pedranzani</LastName><ForeName>H E</ForeName><Initials>HE</Initials></Author><Author ValidYN="Y"><LastName>Sierra de Grado</LastName><ForeName>R</ForeName><Initials>R</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>Argentina</Country><MedlineTA>Biocell</MedlineTA><NlmUniqueID>9438655</NlmUniqueID><ISSNLinking>0327-9545</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D003470">Culture Media</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D002999" MajorTopicYN="N">Clone Cells</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D003470" MajorTopicYN="N">Culture Media</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D046508" MajorTopicYN="N">Culture Techniques</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018514" MajorTopicYN="N">Plant Structures</DescriptorName><QualifierName UI="Q000254" MajorTopicYN="Y">growth & development</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D032107" MajorTopicYN="N">Populus</DescriptorName><QualifierName UI="Q000254" MajorTopicYN="Y">growth & development</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011309" MajorTopicYN="Y">Preservation, Biological</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013030" MajorTopicYN="N" Type="Geographic">Spain</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014197" MajorTopicYN="N">Trees</DescriptorName><QualifierName UI="Q000254" MajorTopicYN="N">growth & development</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2007</Year><Month>8</Month><Day>2</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2007</Year><Month>8</Month><Day>25</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2007</Year><Month>8</Month><Day>2</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">17665638</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Espagne</li></country><region><li>Castille-et-León</li></region></list><tree><noCountry><name sortKey="Pedranzani, H E" sort="Pedranzani, H E" uniqKey="Pedranzani H" first="H E" last="Pedranzani">H E Pedranzani</name><name sortKey="Sierra De Grado, R" sort="Sierra De Grado, R" uniqKey="Sierra De Grado R" first="R" last="Sierra De Grado">R. Sierra De Grado</name></noCountry><country name="Espagne"><region name="Castille-et-León"><name sortKey="Martin, M T" sort="Martin, M T" uniqKey="Martin M" first="M T" last="Martin">M T Martin</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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